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Sczepanski Research Group

Publications

Google Scholar Profile

43. Li, J.; Clark, V.; Yu, C-H.; Scida, K.; Pellitero, M. A.; Albarracín Rivera, R. L.; Zhong, W.; Demek, E.; Fountain, J.; Mahlum, J. D.; Haaland, R. E.; Carr, G. V.; Sczepanski, J. T.* and Arroyo-Currás, N.* Monitoring HIV Antiretroviral Therapy via Aptamer-Based Measurements in Preclinical Animal Models and in Human Plasma. 2024. (In Revision)

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44. Shearer, V.; Yu, C.-H.; Han, X.; Sczepanski, J. T.* The Clinical Potential of L-Oligonucleotides: Challenges and Opportunities. 2024. (In Press)

42. Zhong, W.; Sczepanski, J. T.* Chimeric D/L-DNA Probes of Base Excision Repair Enable Real-Time Monitoring of Thymine DNA Glycosylase Activity in Live Cells. J. Am. Chem. Soc. 2023, 145, 17066-17074.

41. McGregor, L. A.; Deckard, C. E. III; Smolen, J. A.; Porter, G. M.; Sczepanski, J. T.* Thymine DNA Glycosylase Mediates Chromatin Liquid-Liquid Phase Separation in a DNA Methylation-Dependent Manner. Journal of Biological Chemistry 2023, 299, 104907.

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45. Yu, C.-H.; He, X.; Wang, Y.; Sczepanski, J. T.* A Comprehensive Interrogation of Mirror-Image L-RNA-Protein Interactions Reveals Key Mechanisms of L-RNA Cytotoxicity and a Chemical Mitigation Strategy. 2024. (Submitted)

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40. McGregor, L. A.; Zhu, B.; Goetz, A. M.; Sczepanski, J. T.* Thymine DNA Glycosylase is an RNA-Binding Protein with High Selectivity for G-Rich Sequences. Journal of Biological Chemistry 2023, 299, 104590.

39. Yu, C.-H.; Sczepanski, J. T.* The influence of chirality on the behavior of oligonucleotides inside cells: revealing the potent cytotoxicity of G-rich L-RNA. Chemical Science 2023, 14, 1145-1154.

38. Pellitero, M.A.; Kundu, N.; Sczepanski, J.T.; Arroyo-Curras, N.* Os(ii/iii) complex supports pH-insensitive electrochemical DNA-based sensing with superior operational stability than the benchmark methylene blue reporter. Analyst 2023, 148, 806-813.

37. Piwko, T. A.; Han, X.; Kabza, M. A. Dey, S.; Sczepanski, J. T.* Inverse In Vitro Selection Enables Comprehensive Analysis of Cross-Chiral L-Aptamer Interactions. ChemBioChem 2022, 23, e202200520.

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36. Clark, V.; Waters, K.; Orsburn, B.; Bumpus, N. N.; Kundu, N.; Sczepanski, J. T.; Ray, P.; Arroyo-Currás, N.* Human Cyclophilin B Nuclease Activity Revealed via Nucleic Acid-Based Electrochemical Sensors. Angewandte Chemie 2022, 134, e202211292.

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35. Sczepanski, J. T.* L-DNA: Applications and the Recognition of Mirror Image Nucleic Acids. Glen Report 33-21, 2022.

34. Li, J.; Sczepanski, J. T.* Targeting a Conserved Structural Element from the SARS-CoV-2 Genome using L-DNA Aptamers. RSC Chemical Biology 2022, 3, 79-84.

33. Yu, C.-H.; Kabza, A. M.; Sczepanski, J. T.* Assembly of Long L-RNA by Native RNA Ligation. Chem. Commun. 2021, 57, 10508-10511.

32. Kabza, A. M.; Kundu, N.; Zhong, W.; Sczepanski, J. T.* Integration of Chemically Modified Nucleotides with DNA Strand Displacement Reactions for Applications in Living Systems. WIREs Nanomedicine and Nanobiotechnology 2022, 14, e1743 .

31. Kundu, N.; Young, B. E.; Sczepanski, J. T.* Kinetics of Heterochiral Strand Displacement From PNA-DNA Heteroduplexes. Nucleic Acids Res. 2021, 49, 6114-6127.

30. Shaver, A.; Kundu, N.; Young, B. E.; Vieira, P.; Sczepanski, J. T.; Arroyo-Curras, N.* Nuclease Hydrolysis Does Not Drive the Rapid Signaling Decay of DNA Aptamer Based Electrochemical Sensors in Biological Fluids. Langmuir 2021, 37, 5213-5221.

29. Deckard, C. E.; Sczepanski, J. T.* Reversible Chromatin Condensation by the DNA Repair and Demethylation Factor Thymine DNA Glycosylase. Nucleic Acids Res. 2021, 49, 2450-2459.

28. Zhong, W.; Sczepanski, J. T.* Direct Comparison of D-DNA and L-DNA Strand-Displacement Reactions in Living Mammalian Cells. ACS Synth. Biol. 2021, 10, 209-212.

27. Tjhung, K. F.†; Sczepanski, J. T.†; Murtfeldt, E. R.; Joyce, G. J.* RNA-Catalyzed Cross-Chiral Polymerization of RNA. J. Am. Chem. Soc. 2020, 142, 15331–15339.

26. Kabza, A. M.; Sczepanski, J. T.* L-DNA-Based Catalytic Hairpin Assembly Circuit. Molecules 2020, 25, 947. https://doi.org/10.3390/molecules25040947

25. Dey, S.; Sczepanski, J. T.* In Vitro Selection of L-DNA Aptamers that Bind a Structured D-RNA Molecule. Nucleic Acids Res. 2020, 48, 1669–1680.

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24. Young, B. E.; Sczepanski, J. T.* Heterochiral DNA Strand-Displacement Based on Chimeric D/L-Oligonucleotides. ACS Synth. Biol. 2019, 8, 2756–2759.

23. Deckard, C. E.; Banerjee, D. R.; Sczepanski, J. T.* Chromatin Structure and the Pioneering Transcription Factor FOXA1 Regulate TDG-Mediated Removal of 5-formylcytosine from DNA. J. Am. Chem. Soc. 2019, 141, 14110–14114.

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22. Kabza, A. M.; Kundu, N.; Young, B. E.; Sczepanski, J. T.* Heterochiral Nucleic Acid Circuits. Emerg. Top. Life Sci. 2019, 3, 501–506.

21. Banerjee, D. R.; Deckard, C. E. III; Zeng, Y.; Sczepanski, J. T.* Acetylation of the Histone H3 Tail Domain Regulates Base Excision Repair on Higher-Order Chromatin Structures. Sci. Rep. 2019, 9, 15972. https://doi.org/10.1038/s41598-019-52340-0

20. Young, B. E.; Kundu, N.; Sczepanski, J. T.* Mirror-Image Oligonucleotides: History and Emerging Applications. Chem. Eur. J. 2019, 25, 7981–7990.

19. Zhong, W.; Sczepanski, J. T.* A Mirror Image Fluorogenic Aptamer Sensor for Live-Cell Imaging of MicroRNAs. ACS Sens. 2019, 4, 566–570.

18. Deckard, C. E. III; Sczepanski, J. T.* Polycomb Repressive Complex 2 Binds RNA Irrespective of Stereochemistry. Chem. Commun. 2018, 54, 12061–12064.

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17. Banerjee, D. R.†; Deckard, C. E. III †; Elinsky, M. B.; Batteas, J. D.; Sczepanski, J. T.* A Plug-and-Play Approach for Preparing Chromatin Containing Site-Specific DNA Modifications: The Influence of Chromatin Structure on Base Excision Repair. J. Am. Chem. Soc. 2018, 140, 8260–8267.

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16. Kabza, A. M.†; Young, B. E.†; Sczepanski, J. T.* Heterochiral DNA Strand-Displacement Circuits. J. Am. Chem. Soc. 2017, 139, 17715–17718.

15. Kabza, A. M.; Sczepanski, J. T.* An L-RNA Aptamer with Expanded Chemical Functionality Inhibits MicroRNA Biogenesis. ChemBioChem 2017, 18, 1824–1827.

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46. Han, X.; Sczepanski, J. T.* An Expanded Substrate Scope for Cross-Chiral Ligation Enables Efficient Synthesis of Long L-RNAs. bioRxiv (2024), 2024.10.04.616258.

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Before Texas A&M

14. Sczepanski, J. T.; Joyce, G. F.* Specific Inhibition of MicroRNA Processing Using L-RNA Aptamers. J. Am. Chem. Soc. 2015, 137, 16032–16037.

13. Sczepanski, J. T.; Joyce, G. F.* A Cross-Chiral RNA Polymerase Ribozyme. Nature 2014, 515, 440–442.

12. Sczepanski, J. T.; Joyce, G. F.* Binding of a Structured -RNA Molecule by an -RNA Aptamer. J. Am Chem. Soc. 2013, 135, 13290–13293.

11. Zhou, C.; Sczepanski, J. T.; Greenberg, M. M.* Histone Modification via Rapid Cleavage of C4-Oxidized Abasic Sites in Nucleosome Core Particles. J. Am. Chem. Soc. 2013, 135, 5274–5277.

10. Sczepanski, J. T.; Zhou, C.; Greenberg, M. M.* Nucleosome Core Particle-Catalyzed Strand Scission at Abasic Sites. Biochemistry 2013, 52, 2157–2164.

9. Sczepanski, J. T.; Joyce, G. F.* Synthetic Evolving Systems that Implement a User-Specified Genetic Code of Arbitrary Design. Chem. Biol. 2012, 19, 1324–1332.

8. Zhou, C.; Sczepanski, J. T.; Greenberg, M. M.* Mechanistic Studies on Histone Catalyzed Cleavage of Apyrimidinic/Apurinic Sites in Nucleosome Core Particles. J. Am. Chem. Soc. 2012, 134, 16734–16741.

7. Sczepanski, J. T.; Hiemstra, C.; Greenberg, M. M.* Probing DNA Interstrand Cross-Link Formation by an Oxidized Abasic Site Using Nonnative Nucleotides. Bioorg. Med. Chem. 2011, 19, 5788–5793.

6. Sczepanski, J. T.; Wong, R. S.; McKnight, J. N.; Bowman, G. D.; Greenberg, M. M.* Rapid DNA-Protein Cross-Linking and Strand Scission by an Abasic Site in a Nucleosome Core Particle. Proc. Natl. Acad. Sci. USA. 2010, 107, 22475–22480.

5. Wong, R. S.; Sczepanski, J. T.; Greenberg, M. M.* Excision of a Lyase-Resistant Oxidized Abasic Lesion from DNA. Chem. Res. Toxicol. 2010, 23, 766–770.

4.Greenberg, M. M.*; Newman, C. A.; Resendiz, M.; Sczepanski, J. T. Photochemical Generation and Reactivity of 5,6-Dihydrouridin-6-yl Radical. J. Org. Chem. 2009, 74, 7007–7012.

3. Sczepanski, J. T.; Jacobs, A.; Van Houten, B.; Greenberg, M. M.* Double-Strand Break Formation During Nucleotide Excision Repair of a DNA Interstrand Cross-Link. Biochemistry 2009, 48, 7565–7567.

2. Sczepanski, J. T.; Jacobs, A.; Majumdar, A.; Greenberg, M. M.* Scope and Mechanism of Interstrand Cross-Link Formation by the C4-Oxidized Abasic Site. J. Am. Chem. Soc. 2009, 131, 11132–11139.

1. Sczepanski, J. T.; Jacobs, A.; Greenberg, M. M.* Self-Catalyzed DNA Interstrand Cross-Link Formation by an Abasic Site. J. Am. Chem. Soc. 2008, 130, 9646–9647.

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